Journal: PLoS ONE

Article Title: Effects of Negative Pressure Wound Therapy on Mesenchymal Stem Cells Proliferation and Osteogenic Differentiation in a Fibrin Matrix

doi: 10.1371/journal.pone.0107339

Figure Lengend Snippet: Immediately before and 3, 7 days after incubation, cell clots were harvested and confocal microscopy was performed to detect fluorescent immunostaining for COL1. Results showed that the expression of COL1 was absent or weak in two groups before incubation, but stronger in NPWT group at day 3 and 7 after incubation. Bars = 50 µm.

Article Snippet: Then they were blocked with goat serum, and incubated with the specific rat primary antibodies against collagen type 1 (COL1) (1∶100, BOSTER, Wuhan, China) overnight at 4°C.

Techniques: Incubation, Confocal Microscopy, Immunostaining, Expressing

Journal: PLoS ONE

Article Title: Effects of Negative Pressure Wound Therapy on Mesenchymal Stem Cells Proliferation and Osteogenic Differentiation in a Fibrin Matrix

doi: 10.1371/journal.pone.0107339

Figure Lengend Snippet: NPWT-treated and static control group cell clots were harvested before and 1, 3, and 7 days after incubation (n = 3 per timepoint for each group) and analyzed by real-time RT-PCR. The expressions of the specific osteogenic genes (A) ALP, (B) OC, and (D) COL1 were higher in NPWT group than those in control group (* p <0.05, ** p <0.01). The NPWT group also expressed higher levels of the osteogenic transcription factor Cbfa1 (also known as Runx2) and the mechanosignaling molecule integrin β5 (ITGB5) (C) and (E) (* p <0.05, ** p <0.01).

Article Snippet: Then they were blocked with goat serum, and incubated with the specific rat primary antibodies against collagen type 1 (COL1) (1∶100, BOSTER, Wuhan, China) overnight at 4°C.

Techniques: Control, Incubation, Quantitative RT-PCR

Journal: bioRxiv

Article Title: Extracellular matrix guidance determines proteolytic and non-proteolytic cancer cell patterning

doi: 10.1101/2022.03.16.484647

Figure Lengend Snippet: Cell emigration patterns in the presence or absence of MMP inhibitor GM6001 after 48h culture. (A) Xz view of interface migration at indicated conditions and in 6 mg/ml collagen after 48h culture in the presence or absence of GM6001. Where present, the rim of the core is indicated by white dotted line. Graph, COL1 ¾ signal per cell (counted as 75 and 118 nuclei per condition from the invasion zone, but not the core; n=1). (B-G) Cell emigration parameters in the presence of GM6001 after 48h. (A) Gray dotted circles indicate size of depicted spheroid when imaged at 0h culture. (F) Area bordered by white line in top images indicates the area of the zoom-in in the respective lower image; orange arrowheads, interface; open arrowheads, cytoplasmic extensions; closed arrowheads, invaded nuclei. (C-E, G, H) Quantification of interface-guided and non-guided migration parameters as described in and . Data represent 1-4 measurements (dots/line) per condition per experiment (N=2). (I , J) Interface-mediated versus non-guided migration in the absence or presence of MMP activity. (I) Percentage of emigrated cells in the interface compared to total number of emigrated cells per spheroid. (J) Total numbers of interface-guided cells plotted against non-guided cells. Orange line indicates condition of MMP-independent guidance. (I, J) Data from bars depicted with “M” were derived from in silico cellular automata-based model. In vitro data were derived from experiments depicted in ; data represent 1-4 measurements (bar/square) per condition per experiment (N=2). (C-E, H) Solid line, median, (I , J) Bars and squares, mean; whiskers, SD. Mann-Whitney test with, when necessary, Holm-Sidak, ns: not significant; *: P-value < 0.05; **: P-value < 0.01. Scale bars: (A) 50 μm; (B, F) 250 μm.

Article Snippet: Affinity purified rabbit anti-collagen type I cleavage site antibody (Col1¾C short , Immunoglobe), Alexa-488-conjugated secondary pre-absorbed goat anti-rabbit IgG (Invitrogen), DAPI (Sigma) and Alexa-568-conjugated phalloidin (Invitrogen) were used.

Techniques: Migration, Activity Assay, Derivative Assay, In Silico, In Vitro, MANN-WHITNEY